點針細胞電穿孔儀
產品簡介
WPI公司最新上市的點針細胞電穿孔儀是一款簡單、通用的細胞膜或細胞壁的穿透設備， 可用于將DNA、iRNA等遺傳信息物質注入胚胎細胞以及植入前期的哺乳動物胚胎中，可在不撕裂或破壞細胞膜的情況下，實現小創傷、不留痕跡 、精確的膜穿透。
產品特征
● 觸摸屏顯示：電阻式觸摸屏，戴手套也可方便使用; 儀器操作模式：腳踏開關控制注射或觸摸屏手動注射控制 ;
● 直觀的用戶界面;占用空間小;
● 參數設置：通過觸摸屏或旋鈕調節頻率和電壓; 電穿孔程序：四種用戶可編程程序;
● 警示音：可調音頻連續音表示有效探頭; 計數顯示：注射計數器指示注射總次數;
● 通用性：電極支架與大多數常見操縱器兼容; 高存活率：小鼠胚胎雙細胞期注射后約80-85% 存活率;
裝在倒置顯微鏡下的點針細胞電穿孔儀?？梢约嫒軼PI公司SYS-PV830，艾本德公司的FemtoJet注射系統。
這是一個典型的微注射裝置，使用MICRO-ePORE™、一個INV-101倒置顯微鏡和一個PV830氣動皮升操作泵。
產品用途
● 微量注射到卵母細胞和植入前期的哺乳動物胚胎, 包括將CRISPR-Cas9試劑注射到雙細胞胚胎的細胞質中;
● 原核嚙齒動物受精卵微量注射;
● 斑馬魚中的基因沉默;
江西某高校斑馬魚中心使用第二代原型機在斑馬魚尾鰭注入Morphilinos。
參考文獻
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