氣動皮升操作泵
儀器概況
WPI 公司的氣動皮升泵通過使用調節的氣壓來固定細胞并注射液體，是斑馬魚顯微注射及斑馬魚卵細胞基因感染實驗中經典的注射系統。具有使用方便， 注射程序簡單，重復性極佳的優點，注射的體積范圍從pL到nL不等。正壓和負壓由單獨的端口提供，正壓主要用于高壓噴射，負壓用于吸附支持細胞及毛細管尖端的充液，第二個壓力端口在每次注射脈沖之間讓毛細管尖端保持低的正性維持壓，以阻止液體通過毛細管虹吸作用或擴散的方式回吸。
定時作用、噴射壓力、維持壓和吸附作用都是獨立地通過儀器前板的控制旋鈕和顯示表來調節，注射壓通過前板的20圈調節器控制，內置定時電路可以精確控制當注射壓力應用到輸出端口的時間參數。注射壓力間隔可以從前板手動觸發，也可以通過腳踏開關或計算機控制的TTL脈沖觸發，一個5V的輸出為計算機或其它監測設備提供一個邏輯電平脈沖。
SYS-PV830可以通過前板的各自調節器提供噴射壓、維持壓和真空。噴射壓用來提供高壓脈沖注射液體、維持壓主要用來防止毛細管虹吸導致液體尖端回吸。真空也通過前板以同樣的方式調節。真空端口可以在前板用棒狀開關從真空切換到大氣環境、真空可以引導到噴射端口。
產品特征
● 操作簡便
手動控制：輕按前面板啟動鍵開始或停止
腳踏開關：腳踏開關靈活控制開始或停止
外部輸入：通過計算機編程控制時間參數或TTL信號啟動
● 雙重模式
外部啟動：通過計算機控制啟動時間
內部啟動：通過儀器內部晶體振蕩器控制啟動時間
● 計量準確
時間參數：具有100ms和10s兩個時間檔，能夠精確調節到0.2ms
體積準確：通過調節注射時間、注射壓和毛細管尖端大小均可確保注射體積一致
● 可重復性
通過固定壓力、時間和尖端口徑，可確保每次注射體積一致，做到可重復性
產品用途
用于斑馬魚研究：
● 用于斑馬魚卵細胞DNA等遺傳物質注射；
● 用于斑馬魚幼魚體內藥物、染料的微量注射； 用于斑馬魚幼魚體內腫瘤細胞及其它干細胞 的種植；
用于昆蟲研究：
● 用于果繩卵細胞DNA等遺傳物質注射；
● 用于棉鈴蟲、褐飛虱等蠶蛹體內藥物及染料等微量注射；
用于嗅覺或味覺研究：
● 用過PUFF方式對神經元或腦內核團給刺激物或氣味；
用于物質的轉移：
● 用于細胞核或細胞器移植； 用于微小粒子的移??；
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